Incisional and Excisional Biopsy

The formalin-fixed and hematoxylin- and eosin-stained sections showed evidence of supra-basilar epithelial separation (Fig 2) with acantholysis (Fig 3) releasing a few Tzanck cells. The immunofluorescence antibody stain revealed positive staining with IgG (Fig 4) and C3 (Fig 5) antibodies in arranged in a fishnet pattern within the spinous layer cells.

Figure 2. Low power (x100) view of a hematoxylin and eosin stained tissue section demonstrating a clear supra basilar epithelial split.

 

Figure 3. Low power (x100) view of a hematoxylin and eosin stained tissue section demonstrating a supra basilar epithelial split and acantholysis.

Fig 4. Low power (x200) view of a direct Immunoflourescent stain demonstrating positive staining with IgG antibody with a fishnet appearance.

Fig 5. Low power (x100) view of a direct Immunoflourescent stain demonstrating positive staining with C3 antibody with a fishnet appearance.