Incisional and excisional biopsy

The biopsy material was processed for immunofluoresnce and H & E stains. The latter showed evidence of keratinized and proliferative surface epithelium with a basal cell layer exhibiting focal degeneration (Figures 3 & 4). The superficial connective tissue was infiltrated by many lymphocytes arranged in a linear fashion. The immunofluoresence stain was negative with IgG, IgA, IgM and C3 antibodies. Positive stain was identified with antibody to fibrinogen (Figure 5) present along the basement membrane in a linear fashion.

Figure 3 Screening power (40X) H & E histology shows a fragment of mucosa covered by keratinized epithelium supported by fibrous connective tissue containing a band-like infiltrate of lymphocyte.

 

Figure 4 Higher power (200X) H & E histology of the surface epithelium exhibiting basal cell degeneration with lymphocytes arranged directly beneath the surface epithelium.

Figure 5 Low power (100X) immunofluoresence histology of the surface epithelium exhibiting a uniformly positive line of fibrinogen along the basement membrane.